What are the steps for DNA amplification process/experiment?

Please help me
Thanks

Answers

Answer 1
(1) denaturation of the template into single strands; (2) annealing of primers to each original strand for new strand synthesis; and (3) extension of the new DNA strands from the primers.

Hope this helps! :)

Related Questions

What happens is a cell does not recognize something

Answers

Answer:

Generally, if a cell does not recognize something, it will launch an immune response.

Explanation:

This is why individuals who have organ transplants must be put on immunosuppressants, when cells do not recognize a foreign body they will immediately assume it is harmful and make an attempt to attack it.

In what ways do viruses differ from other pathogens?.

Answers

Viruses differ from other pathogens because they are considered non-living.

What are viruses?

Viruses are organisms that are incapable of reproducing on their own without a living host.

Viruses cannot undergo any of the characteristics of life outside a living host.

They, therefore, infect their host and use the mechanisms of the host to replicate themselves.

Therefore, viruses differ from other pathogens because they are considered non-living.

Learn more about viruses at: https://brainly.com/question/13619671

Which of the following best describes the total amount of carbon in the system made up of the Earth and its atmosphere?
A. It remains constant because oxygen converts to carbon in the geosphere and hydrosphere.
B. It continually increases as carbon is produced during cellular respiration and trapped in the atmosphere.
C. It continually decreases as carbon is used up during photosynthesis and trapped in the biosphere.
D. It remains constant because carbon cycles through the atmosphere, hydrosphere, biosphere, and geosphere.

Answers

Answer:

A

Explanation:

It remains constant because carbon cycles through the atmosphere, hydrosphere, biosphere, and geosphere.

Assuming that the population was in hardy-weinberg equilibrium for the g locus, what percentage of moths in the natural population was white in 1962 ?.

Answers

The percentage of moths in the natural population that was white in 1962 is : 4%

What is Hardy-weinberg equilibrium ?

Hardy-weinberg equilibrium states that the sum total of all the genes of an individual in a population is constant when the disturbing factors such as genetic drift does not occur

p² + q² + 2pq = 1

Determine the percentage of moths in 1962

Applying the equation below

p² + q² + 2pq = 1

p = frequency of dominant allele

q = frequency of recessive allele

q² = frequency of h0m0zygous recessive genotype

p² = frequency of h0m0zygous dominant genotype

From the graph

Frequency of G allele ( i.e p ) = 0.8

percentage of white moths = q²

Note : p + q = 1  ---- ( 1 )

Insert value into equation ( 1 )

0.8 + q = 1

therefore ; q = 0.2

Hence the percentage of white moths = ( 0.2 )² = 0.04

                                                                               = 4%

Hence we can conclude that The percentage of moths in the natural population that was white in 1962 is : 4%

Learn more about hardy-weinberg equilibrium : https://brainly.com/question/3406634

Which of the following is/are most likely to be part of the gastrointestinal reflex pathway? 1. Stretch receptors 2. Neurons of the autonomic nervous system 3. Muscle fibers of the transversus abdominus.

Answers

The stretch receptors and neurons of the autonomic nervous system are parts of the gastrointestinal reflex pathway.

What is Gastrointestinal reflex?

This is usually stimulated by senses as a result of the presence of stretch receptors and neurons of the autonomic nervous system.

They help in controlling  the release of acids in the stomach and also suppresses the release of digestive proteins.

Read more about Gastrointestinal reflex here https://brainly.com/question/1386870

Clostridium tetani is a bacterium that can be found in soil.

It causes the infection tetanus.

Chrildren are vaccinated against tetanus.

Explain why these children do not get tetanus if the bacteria enters their body through a cut in the skin.

Answers

Explanation:

because vaccine injected to them produces antibodies which fight against the bacteria

I need help I don't understand

Answers

they may look different because one slide may be dyed and one slide may not. or they may b slides of different things

What is the climate and vegetation like in the temperate woodland?

Answers

Answer:

woodlands and lush vegetation

Explanation

Deciduous woodlands contain trees with broad leaves such as oak, beech and elm. They occur in places with high rainfall, warm summers and cooler winters and lose their leaves in winter.

Answer:

high rainfall, warm summers and cooler winters

the landscape contains  oak, beech, elm, Lichen, moss, ferns, wildflowers and other small plants

The temperature in a temperate woodland & shrubland biome is hot & dry in the summer with temperatures up to 100 degrees F. The winter is cool & moist with the low temperature about 30 degrees F. Precipitation: Annual rainfall is 10-17 in.  

Hope This Helps

Could the world's
Cavendish banana crop be
destroyed some day?
Construct an explanation to
support your answer. Base
the explanation on evidence
from the potato famine.

Answers

Cavendish banana crop be destroyed some day due to Panama disease.

How Cavendish banana crop be destroyed?

The Cavendish banana is considered as the most popular banana worldwide but it could disappear in the coming years because of the attack of an aggressive fungus called "Panama Disease."

This disease destroy the banana plant at faster rate which leads to its destruction so we can conclude that Cavendish banana crop be destroyed some day due to Panama disease.

Learn more about Cavendish here: https://brainly.com/question/13165684

How many dna molecules are in three pair of homologous chromosomes

Answers

Answer:

Thus during metaphase of mitosis, each chromosome (i.e., each chromatid pair) will contain two molecules

Explanation:

double stranded DNA (one molecule per sister chromatid). The following diagram depicts the behavior of one homologous pair of chromosomes during mitosis.

Six DNA molecules are there in three pairs of homologous chromosomes. This is because each homologous chromosome will contain two molecules of double-stranded DNA.  

What do you mean by Homologous chromosomes?

Homologous chromosomes may be defined as two chromosomes of the same length, position, and structure one inherited from the father's side and the other from the mother's side.

The amount of genetic material may vary from phase to phase of the cell cycle. But at the time of metaphase during mitosis, each chromosome will possess two molecules of DNA.

Therefore, Six DNA molecules are there in three pairs of homologous chromosomes.

To learn more about Homologous chromosomes, refer to the link;

https://brainly.com/question/8253366

#SPJ4

Which explains how the economic situation in chile in 1980 affected its government? chile’s improving economy led to the government overthrowing a military junta. chile’s worsening economy led to the government electing a socialist president. chile’s worsening economy led to the government being overthrown by a military junta. chile’s improving economy led to the government launching populist programs.

Answers

The economic situation best describe by Chile’s worsening economy led to the government being overthrown by a military junta.

What is the economic history of Chile?

The economic condition of Chile changed over time.

Due to a worldwide recession, the GDP of Chile dropped to 14.1 in 1980.

The value of the currency is overvalued.

Due to the Asian crisis, in 1997 economic growth depletion started.

Thus, the correct option is C.

Learn more about  Chile

https://brainly.com/question/14215140

Answer:

C.

Chile’s worsening economy led to the government being overthrown by a military junta.

Explanation:

yes

In the Hardy-Weinberg principle, the actual number values of p and q will not change if five specific conditions are being met. This indicates

Question 1 options:

evolution is occurring only because of mutations


evolution is not occuring


evolution is occurring rapidly because of climate change


evolution is occurring because of natural selection

Answers

In the Hardy-Weinberg principle, the values of p and q will not change if evolution is not occurring.

What is the Hardy-Weinberg principle?

The Hardy-Weinberg principle is a model used in population genetics to estimate genotypic and allele frequencies in a population.

The Hardy-Weinberg principle states that allele and genotypic frequencies remain constant in absence of evolutionary forces.

These evolutionary forces include nonrandom mating, gene drift, gene flow, mutation and natural selection.

Learn more about the Hardy-Weinberg principle here:

https://brainly.com/question/3406634

A species of squirrel has skin cells that contain 20 chromosomes. How many chromosomes would be found in the sperm produced by heat same squirrel?

Answers

Answer:

40

Explanation:

40 chromosomes would be found in the sperm produced by heat same squirrel

40 chromosomes would be found in the sperm produced by heat same squirrel.

What are Chromosomes?

A chromosome is a lengthy DNA molecule that contains all or a portion of an organism's genetic code. The very long, thin DNA fibres in most chromosomes are covered with packing proteins; in eukaryotic cells, the histones are the most significant of these proteins.

The highest possible level of DNA and protein organisation is seen in chromosomes. Chromosomes' primary job is to carry DNA and pass genetic material from one generation to the next. Cell division involves the critical function of chromosomes. They guard against tangles and damage to the DNA.

For above given example, if 20 chromosomes are present in skin cells, then the squirrel will have 40 chromosomes in the sperm cells.

Thus, 40 chromosomes would be found in the sperm produced by heat same squirrel.

Learn more about chromosomes, here:

https://brainly.com/question/30993611

#SPJ2

With your group, list at least three possible uses and/or products of the energy absorbed by the oak leaves that did not contribute to the production of biomass

Answers

Answer:

heat loss, energy for cellular process: transpiration, other forms of water, oxygen, carbon dioxide loss

Explanation:

The energy received by the Oak leaves which did not contribute to the synthesis of biomass may be used for or produced as heat loss, energy for cellular processes such as transpiration, other forms of water, oxygen, or carbon dioxide loss.

What is Biomass?

The material from previously living organisms known as biomass is utilised to produce bioenergy. Examples include wood, wood byproducts, energy crops, agricultural waste, and organic waste from commercial and residential sources. The main biomass energy source nowadays is wood and wood waste.

Renewable organic material from plants and animals is known as biomass. Up until the middle of the 1800s, biomass accounted for the majority of the entire yearly U.S. energy consumption. Biomass is a popular fuel in many nations, particularly for heating and cooking in underdeveloped nations.

Thus, the energy received by the Oak leaves which did not contribute to the synthesis of biomass may be used for or produced as heat loss, energy for cellular processes such as transpiration, other forms of water, oxygen, or carbon dioxide loss.

Learn more about Biomass, here:

https://brainly.com/question/14122480

#SPJ2

A town’s population size has been slowly increasing for the past five years. The town’s total electricity use has also slowly increased, until last year when it decreased slightly. Explain what could have caused the decrease in the town’s total electricity use.

Answers

The reason why the electricity could decrease is because the population of the town’s people has gone down causing the electricity rate to decrease. Hope this helps you.

Approximately
% of the available energy is passed from organisms at one trophic level to organisms at the next trophic level.

Answers

The answer is about 10 percent

Approximately 10% of the available energy is passed from organisms at one trophic level to organisms at the next trophic level. This is known as the 10% rule or the 10% energy transfer efficiency.

What is 10% rule?

Energy flows through trophic levels in an ecosystem starting with producers (plants or algae) that convert sunlight into chemical energy through photosynthesis. When herbivores consume these producers, only a fraction of the energy stored in the plants is assimilated into their bodies, while the rest is lost as waste or used for metabolic processes like respiration. This assimilated energy becomes available to the next trophic level.

As energy moves up the food chain, similar inefficiencies occur at each level. Carnivores that consume herbivores receive only about 10% of the energy stored in their prey. This pattern continues as energy is transferred to higher-level predators.

The low energy transfer efficiency can be attributed to several factors. First, organisms expend energy for basic metabolic processes, such as respiration, movement, and growth, which reduces the energy available for transfer. Second, energy is lost as heat during these metabolic processes. Additionally, not all parts of an organism are consumed or digested by predators, resulting in energy losses through waste.

Learn more about trophic level, here:

https://brainly.com/question/13267084

#SPJ5

How is preimplantation genetic diagnosis used in the future?

Please help
Thank you

Answers

Answer:

In recent years PGD technology has been increasingly used by infertile patients undergoing IVF treatment, in order to screen their embryos for chromosomal abnormalities. Aneuploidy is extremely common in human embryos and leads to a developmental arrest, implantation failure and spontaneous abortion.

What is the proper name of the plasma membrane found on a muscle fiber?

Answers

Answer:

Answered below

Explanation:

the plasma membrane of a skeletal muscle fiber is called the sarcolemma. The sarcolemma invaginates into the cytoplasm of the muscle cell (sarcoplasm). This forms membranous tubules that pass across the muscle cells.

Which molecule, chlorophyll a or chlorophyll b, is more polar?.

Answers

Answer:

Molecular Structure and Polarity. The distinctions between the chlorophylls, which are more polar than β-carotene are slight: chlorophyll a has a methyl group (Y=CH3) in a position where chlorophyll b has an aldehyde (Y=CHO). This makes chlorophyll B slightly more polar than chlorophyll A.

Determine whether each phrase mainly describes surface ocean currents or deep ocean currents. found near the top of the ocean caused by prevailing winds caused by differences in water density caused by differences in water temperature influenced by rotation of earth found closer to the ocean floor

Answers

Influenced by rotation of Earth

caused by prevailing winds

found near the top of the ocean

The phrases that describe deep Ocean currents are :

caused by differences in water

found closer to the ocean floor

caused by differences in water density

Surface ocean currents are found near the top of the ocean, and they are caused by prevailing winds. The wind creates a force on the surface of the ocean, which causes water to move in the direction of the wind.

What are these currents responsible for ?

These currents are responsible for redistributing heat around the planet, making them an important factor in regulating climate.

Deep ocean currents, on the other hand, are caused by differences in water density and temperature. Cold, dense water sinks to the bottom of the ocean, creating a current that can move across the ocean floor.

This process is known as thermohaline circulation, and it helps to redistribute nutrients and oxygen throughout the ocean, making it an important factor in marine ecosystems.

The rotation of the earth also influences ocean currents. Due to the Coriolis effect, surface currents in the Northern Hemisphere tend to flow clockwise, while currents in the Southern Hemisphere tend to flow counterclockwise. This effect is caused by the rotation of the earth and the resulting centrifugal force.

Finally, currents that are found closer to the ocean floor are deep ocean currents. These currents are slower and less visible than surface currents but can have a significant impact on the ocean's circulation and the Earth's climate.

Learn more about currents  at :

https://brainly.com/question/13076734

#SPJ5

People once believed that all planets and stars orbited around
a. Mercury
C. Earth
b. Venus
d. Mars

Answers

People once believed that all planets orbited around Earth

What is the control group of plants can hear music or sound

Answers

Answer:

Wht are the answer choices

Explanation:

How can you relate the structures
to those of bryophytes in terms of evolutionary trend

Answers

Answer:

Because ferns and fern allies posses true vascular tissues, they can grow to be much larger and thicker than the bryophytes.

Bryophytes mark the transition to land and the origin of vascular plants, and hence, link the seed and vascular plants to their algal ancestors.

Molecular phylogenetic studies conclude that bryophytes are the earliest diverging lineages of the extant land plants. They provide insights into the migration of plants from aquatic environments to land.

What is evolution trends?

Evolutionary trends, whether macro evolutionary events across higher taxa and large spans of geologic time, or more circumscribed events within smaller clades, have long played a prominent role in evolutionary theory.

The identification of trends in the history of life has been used as evidence for the increasing adaptions of life, for evolutionary progress, and more recently as support for selection at multiple levels.

To know more about evolution here

https://brainly.com/question/13492988

#SPJ2

Which of the following best explains why conserving resources is important?

Answers

Answer:

we should listen to our elders

Explanation:

resources is important

What important role does water play in the function that needs to be performed by the kidneys​

Answers

Answer:

Water helps the kidneys remove waste from your blood through urine. Water also helps keep blood vessels open, which lets blood flow through easier, letting the blood remove its waste through the kidney more efficiently.

Explanation:

Answer:

It helps in Osmoregulation and removal of excess liquid from the body.

Explanation:

It regulates fluid balance by excreting excess amount of water as urine while retaining the necessary amount of water in the body that is essential for living.

Osmoregulation - the process of maintaining salt and water/osmotic balance across membranes within the body.

The fluids inside and surrounding cells are composed of water, electrolytes, and non-electrolytes.

true or false condensation occurs ehen water turns from a liquid to a gas?​

Answers

Answer:

false

Explanation:

Which is a correct observation based on the photo shown below?

A bird with green, yellow, black, and white feathers is shown. It has a long narrow beak that is longer than the length of its head.
The bird’s coloring indicates that it lives in trees with green and yellow leaves.
The bird has a narrow beak that is longer than the length of its head.
The bird probably uses its long beak to reach the nectar inside flowers.
The bird must inhabit a cold climate since there is ice on the branches.

Answers

Answer:

The bird has a narrow beak that is longer than the length of its head.

Explanation:

-The beak is long and thin, which helps it dip into the flowers to sip nectar (a sugary fluid the hummingbird uses for food).

-This bird (Hummingbird) have a long, narrow beak that allows it to reach the nectar from brightly colored, tubular flowers. It also feeds on insects and have a flexible lower beak that allows them to grab insects from the air during flight.

hope that helps :)

Answer:

The bird has a narrow beak that is longer than the length of its head

Explanation:

-The beak is long and thin, which helps it dip into the flowers to sip nectar (a sugary fluid the hummingbird uses for food).

-This bird (Hummingbird) have a long, narrow beak that allows it to reach the nectar from brightly colored, tubular flowers. It also feeds on insects and have a flexible lower beak that allows them to grab insects from the air during flight.

hope that helps :)

How many bivalents are formed in a cell with 20 chromosomes at the beginning of meiosis i?.

Answers

There are 10 bivalents formed in a cell with 20 chromosomes at the beginning of meiosis I

Inadequate blood flow to the heart muscle can cause an area of damaged cardiac tissue known as a(n) .

Answers

A myocardial infarction comprises a series of changes in the cardiac tissue that originate when there is an inadequate blood flow to the heart muscle or a coronary artery becomes blocked.

What is a myocardial infarction?

It is characterized by the sudden appearance of a picture of ischemic suffering (lack of irrigation) to a part of the heart muscle produced by the acute and total obstruction of one of the coronary arteries.

It is recognized by the sudden appearance of the characteristic symptoms of intense pain in the chest, in the precordial area and a feeling of general malaise.

Therefore, we can conclude that a myocardial infarction comprises a series of changes in the cardiac tissue that originate when there is an inadequate blood flow to the heart muscle.

Learn more about a myocardial infarction here: https://brainly.com/question/15319337

Describe the importance of the nucleus membrane being part of the endoplasmic
reticulum and the Golgi apparatus' membranes

Answers

Answer: Nucleus membrane isn't part of the ER or Golgi Apparatus, BUT all of them are part of the endomembrane system.

Endoplasmic reticulum and Golgi apparatus are two membrane-bound organelles found in eukaryotes. Both these organelles are closely associated and functionally related. Both endoplasmic reticulum (ER) and Golgi apparatus are two components of the endomembrane system of a cell. Lysosomes and vesicals are the other components of the endomembrane system.

Other Questions
What do the following words have in common? ACT COAT DOG FOOT LINE PIN STAND the earth exerts a gravitational force of 850N on John. What is Johns mass in kg Is 206 3x) + x equivalent to 2(3x) + x + 12? Where in the United States and what year was the first documented case?West Nile virus! please help me 17What percent does 208%850%85% What is the equation of a circle with center (5, 8) and radius 2?(x 5)2 + (y 8)2 = 4(x + 5)2 + (y + 8)2 = 4(x + 5)2 + (y + 8)2 = 2(x 5)2 + (y 8)2 = 2 What volume of a 0. 120 m cai2 solution would contain 0. 078 mol of the solute?. Donetta is investing $6000 in a stock fund that is offering a constant annual interest of 5%. Briefly explain ONE argument that supports the view that the Spanish-American War was caused by imperialist motives. The circumference of the hub cap of a tire is 77.62 centimeters. Find the area of this hub cap. Use3.14 for it.PLEASE HELP ME The repetition of phrases or sentences with similar structures or meanings iscalled____.A. anaphoraB. dictionC. onomatopoeia I took the Integrity Pledge.Do I put True or False ?True or FalseWhat does integrity mean? Allie and Roman are now trying to graph the red line shown here.Allie entered this equation: y=-2Roman entered this equation: x=-2Who is right, and how do you know? Which of these can be a problem for people with accessibility issues? Select 4 options. using left-aligned textusing an image instead of textusing all uppercase textputting two spaces after a periodflashing images PLEASE HELP 100 POINTS!!!Identify the vertex of the function graphed below. A. (1,1) B. (2,2) C. (3,1) D. (0,-2) brainliest if it is right The painting above uses light to create the main focus on (2x) (x-1)(x-4)=40 pls simplify What is a limitation of a primary-source argument?relevant factspersonal opinions verifiable informationconvincing conclusions Read the following scene from the inheritors. Fred jordan's cell. Slowly, at the end left unchalked, as for a door, she goes in. Her hand goes up as against a wall; looks at her other hand, sees it is out too far, brings it in, giving herself the width of the cell. Walks its length, halts, looks up. ) and one windowtoo high up to see out. (in the moment she stands there, she is in that cell; she is all the people who are in those cells. Emil johnson [who works at the courthouse], appears from outside. ) madeline: (stepping out of the cell door, and around it) hello, emil. Emil: how are you, madeline? how do, mr morton. (ira barely nods and does not turn. Emil turns back to madeline) well, i'm just from the courthouse. Looks like you and i might take a ride together, madeline. You come before the commissioner at four. A possible disadvantage to watching this scene, as opposed to reading it silently, is that viewers are required to conceptualize the setting of the jailhouse. Required to interpret movements in absence of stage directions. Required to imagine the appearance of the characters. Required to visualize the props used within the scene