The ADG1(ADP glucose pyrophosphorylase 1) is involved in flowering timing in plants. You are interested in developing a DNA probe using partial protein sequence information. Part of the amino acid sequence is Glu-Glu-His-Asp-Val-Leu 1. determine the possible antisense oligo nucleotides possible from this amino acid sequence 2. How many total unique different oligonucleotide would need to be produced to cover all possibilities
Answers
Answer:
1) UUC-AUG-AUC-AAG-UAA.
2) 48
Explanation:
1) Determine the possible antisense oligo nucleotides possible from this amino acid sequence
Given that the peptide sequence is = Glu-Glu-His-Asp-Val-Leu 1.
we will use a codon table to show the codons and Anticodons for each of the Amino acids
Amino Acid Codons Anticodons
Glu GAA, GAG UUC, CUC
His CAU, CAC AUG, GUG
Asp GAU, GAC AUC, GUC
Val GUU, GUC, GUA, GUG AAG, GAC, UAC,CAC
Leu UUA, UUG, CUU, CUC, CUA, CUG | UAA, CAA, AAG, GAG,UAG,CAG
From the table above
The possible antisense oligo nucleotide sequence is : UUC-AUG-AUC-AAG-UAA.
2) How many total unique different oligonucleotide would need to be produced to cover all possibilities
A desired peptide sequence requires 16 anticodons
Hence the total unique different oligonucleotide needed to be produced to cover all possibilities = 16 *3 = 48
The ADG1(ADP glucose pyrophosphorylase is involved in flowering timing in plants and a DNA probe is made by using partial protein sequence information. Part of the amino acid sequence is Glu-Glu-His-Asp-Val-Leu.
1. To determine the possible antisense oligonucleotides possible from this amino acid sequence:
Since, for every amino acid, there is a specific codon and anticodons are present so according to the given information, the possible antisense oligonucleotides for Glu-Glu-His-Asp-Val-Leu is UUC-AUG-AUC-AAG-UAA.
2) The total number of unique different oligonucleotides that would need to be produced to cover all possibilities is 48
since the amino acid sequence is Glu-Glu-His-Asp-Val-Leu has 16 anticodons and each anticodon have 3 nucleotides so the possible number of oligonucleotides are :
16×3 = 48.
What are codon and anticodon?A codon is a trinucleotide sequence of RNA that corresponds to a specific amino acid.
An anticodon is a trinucleotide sequence complementary to that of a corresponding codon in a messenger RNA sequence.
Thus, according to codon and anticodon, the possible antisense oligonucleotides for Glu-Glu-His-Asp-Val-Leu is UUC-AUG-AUC-AAG-UAA and a number of unique different oligonucleotides that would need to be produced to cover all possibilities is 48.
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